Production of eicosapentaenoic acid (EPA, 20:5n-3) in transgenic peanut (Arachis hypogaea L.) through the alternative Δ8-desaturase pathway.
Identifieur interne : 000408 ( Main/Exploration ); précédent : 000407; suivant : 000409Production of eicosapentaenoic acid (EPA, 20:5n-3) in transgenic peanut (Arachis hypogaea L.) through the alternative Δ8-desaturase pathway.
Auteurs : Chenchen Wang [République populaire de Chine] ; Xiaohe Qing [République populaire de Chine] ; Mingli Yu [République populaire de Chine] ; Quanxi Sun [République populaire de Chine] ; Fengzhen Liu [République populaire de Chine] ; Baoxiu Qi [Royaume-Uni] ; Xinzheng Li [République populaire de Chine]Source :
- Molecular biology reports [ 1573-4978 ] ; 2019.
Descripteurs français
- KwdFr :
- Acide eicosapentanoïque (analogues et dérivés), Acide eicosapentanoïque (biosynthèse), Acide eicosapentanoïque (génétique), Acide eicosapentanoïque (métabolisme), Arachis (génétique), Chromatographie en phase gazeuse (méthodes), Fatty acid desaturases (génétique), Feuilles de plante (métabolisme), Graines (métabolisme), Ingénierie des protéines (méthodes), Oxidoreductases (génétique), Oxidoreductases (métabolisme), Protéines recombinantes (biosynthèse), Végétaux génétiquement modifiés (génétique).
- MESH :
- analogues et dérivés : Acide eicosapentanoïque.
- biosynthèse : Acide eicosapentanoïque, Protéines recombinantes.
- génétique : Acide eicosapentanoïque, Arachis, Fatty acid desaturases, Oxidoreductases, Végétaux génétiquement modifiés.
- métabolisme : Acide eicosapentanoïque, Feuilles de plante, Graines, Oxidoreductases.
- méthodes : Chromatographie en phase gazeuse, Ingénierie des protéines.
English descriptors
- KwdEn :
- Arachis (genetics), Chromatography, Gas (methods), Eicosapentaenoic Acid (analogs & derivatives), Eicosapentaenoic Acid (biosynthesis), Eicosapentaenoic Acid (genetics), Eicosapentaenoic Acid (metabolism), Fatty Acid Desaturases (genetics), Oxidoreductases (genetics), Oxidoreductases (metabolism), Plant Leaves (metabolism), Plants, Genetically Modified (genetics), Protein Engineering (methods), Recombinant Proteins (biosynthesis), Seeds (metabolism).
- MESH :
- chemical , analogs & derivatives : Eicosapentaenoic Acid.
- chemical , biosynthesis : Eicosapentaenoic Acid, Recombinant Proteins.
- genetics : Arachis, Eicosapentaenoic Acid, Fatty Acid Desaturases, Oxidoreductases, Plants, Genetically Modified.
- chemical , metabolism : Eicosapentaenoic Acid, Oxidoreductases, Plant Leaves, Seeds.
- methods : Chromatography, Gas, Protein Engineering.
Abstract
An important alternative source of fish oil is its production by plants through metabolic engineering. To produce eicosapentaenoic acid (EPA, 20:5n-3) in peanut through the alternative Δ8-pathway, a plant expression vector containing five heterologous genes driven by the constitutive 35S promoter respectively, namely, ∆9-elongase (Isochrysis galbana), ∆8-desaturase (Euglena gracilis), ∆5-desaturase (Mortierella alpina), ∆15-desaturase (Arabidopsis thaliana) and ∆17-desaturase (Phytophthora infestans) were transferred into peanut through Agrobacterium-mediated transformation method. The gas chromatography results indicated that the average content of EPA in the leaves of the transgenic lines was 0.68%, and the highest accumulation of EPA in an individual line reached 0.84%. This finding indicates that it is feasible to synthesize EPA in peanut through metabolic engineering and lays the foundations for the production of very-long-chain polyunsaturated fatty acids (VLCPUFAs) in peanut seeds.
DOI: 10.1007/s11033-018-4476-1
PubMed: 30511300
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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first="Chenchen" last="Wang">Chenchen Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Qing, Xiaohe" sort="Qing, Xiaohe" uniqKey="Qing X" first="Xiaohe" last="Qing">Xiaohe Qing</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Mingli" sort="Yu, Mingli" uniqKey="Yu M" first="Mingli" last="Yu">Mingli Yu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Quanxi" sort="Sun, Quanxi" uniqKey="Sun Q" first="Quanxi" last="Sun">Quanxi Sun</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Peanut Research Institute, Qingdao, 266100, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Peanut Research Institute, Qingdao, 266100, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Fengzhen" sort="Liu, Fengzhen" uniqKey="Liu F" first="Fengzhen" last="Liu">Fengzhen Liu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Agronomy, Shandong Agricultural University, Taian, 271000, Shandong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agronomy, Shandong Agricultural University, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Qi, Baoxiu" sort="Qi, Baoxiu" uniqKey="Qi B" first="Baoxiu" last="Qi">Baoxiu Qi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY</wicri:regionArea><wicri:noRegion>BA2 7AY</wicri:noRegion></affiliation></author><author><name sortKey="Li, Xinzheng" sort="Li, Xinzheng" uniqKey="Li X" first="Xinzheng" last="Li">Xinzheng Li</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China. lxz@sdau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China. lxz@sdau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular biology reports</title><idno type="eISSN">1573-4978</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arachis (genetics)</term><term>Chromatography, Gas (methods)</term><term>Eicosapentaenoic Acid (analogs & derivatives)</term><term>Eicosapentaenoic Acid (biosynthesis)</term><term>Eicosapentaenoic Acid (genetics)</term><term>Eicosapentaenoic Acid (metabolism)</term><term>Fatty Acid Desaturases (genetics)</term><term>Oxidoreductases (genetics)</term><term>Oxidoreductases (metabolism)</term><term>Plant Leaves (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Protein Engineering (methods)</term><term>Recombinant Proteins (biosynthesis)</term><term>Seeds (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide eicosapentanoïque (analogues et dérivés)</term><term>Acide eicosapentanoïque (biosynthèse)</term><term>Acide eicosapentanoïque (génétique)</term><term>Acide eicosapentanoïque (métabolisme)</term><term>Arachis (génétique)</term><term>Chromatographie en phase gazeuse (méthodes)</term><term>Fatty acid desaturases (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Graines (métabolisme)</term><term>Ingénierie des protéines (méthodes)</term><term>Oxidoreductases (génétique)</term><term>Oxidoreductases (métabolisme)</term><term>Protéines recombinantes (biosynthèse)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Eicosapentaenoic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Eicosapentaenoic Acid</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acide eicosapentanoïque</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Acide eicosapentanoïque</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arachis</term><term>Eicosapentaenoic Acid</term><term>Fatty Acid Desaturases</term><term>Oxidoreductases</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Acide eicosapentanoïque</term><term>Arachis</term><term>Fatty acid desaturases</term><term>Oxidoreductases</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Eicosapentaenoic Acid</term><term>Oxidoreductases</term><term>Plant Leaves</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Gas</term><term>Protein Engineering</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide eicosapentanoïque</term><term>Feuilles de plante</term><term>Graines</term><term>Oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie en phase gazeuse</term><term>Ingénierie des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An important alternative source of fish oil is its production by plants through metabolic engineering. To produce eicosapentaenoic acid (EPA, 20:5n-3) in peanut through the alternative Δ8-pathway, a plant expression vector containing five heterologous genes driven by the constitutive 35S promoter respectively, namely, ∆9-elongase (Isochrysis galbana), ∆8-desaturase (Euglena gracilis), ∆5-desaturase (Mortierella alpina), ∆15-desaturase (Arabidopsis thaliana) and ∆17-desaturase (Phytophthora infestans) were transferred into peanut through Agrobacterium-mediated transformation method. The gas chromatography results indicated that the average content of EPA in the leaves of the transgenic lines was 0.68%, and the highest accumulation of EPA in an individual line reached 0.84%. This finding indicates that it is feasible to synthesize EPA in peanut through metabolic engineering and lays the foundations for the production of very-long-chain polyunsaturated fatty acids (VLCPUFAs) in peanut seeds.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30511300</PMID><DateCompleted><Year>2019</Year><Month>07</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-4978</ISSN><JournalIssue CitedMedium="Internet"><Volume>46</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Molecular biology reports</Title><ISOAbbreviation>Mol Biol Rep</ISOAbbreviation></Journal><ArticleTitle>Production of eicosapentaenoic acid (EPA, 20:5n-3) in transgenic peanut (Arachis hypogaea L.) through the alternative Δ8-desaturase pathway.</ArticleTitle><Pagination><MedlinePgn>333-342</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11033-018-4476-1</ELocationID><Abstract><AbstractText>An important alternative source of fish oil is its production by plants through metabolic engineering. To produce eicosapentaenoic acid (EPA, 20:5n-3) in peanut through the alternative Δ8-pathway, a plant expression vector containing five heterologous genes driven by the constitutive 35S promoter respectively, namely, ∆9-elongase (Isochrysis galbana), ∆8-desaturase (Euglena gracilis), ∆5-desaturase (Mortierella alpina), ∆15-desaturase (Arabidopsis thaliana) and ∆17-desaturase (Phytophthora infestans) were transferred into peanut through Agrobacterium-mediated transformation method. The gas chromatography results indicated that the average content of EPA in the leaves of the transgenic lines was 0.68%, and the highest accumulation of EPA in an individual line reached 0.84%. This finding indicates that it is feasible to synthesize EPA in peanut through metabolic engineering and lays the foundations for the production of very-long-chain polyunsaturated fatty acids (VLCPUFAs) in peanut seeds.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Chenchen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qing</LastName><ForeName>Xiaohe</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Mingli</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Quanxi</ForeName><Initials>Q</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-4075-1955</Identifier><AffiliationInfo><Affiliation>Shandong Peanut Research Institute, Qingdao, 266100, Shandong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Fengzhen</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Agronomy, Shandong Agricultural University, Taian, 271000, Shandong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Baoxiu</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xinzheng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271000, Shandong, China. lxz@sdau.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Life Science, Shandong Agricultural University, Daizong Road No. 61, Taian, 271000, Shandong, China. lxz@sdau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>31271757</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>2016KF09</GrantID><Agency>National Opening Project of State Key Laboratory of Crop Biology</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Mol Biol Rep</MedlineTA><NlmUniqueID>0403234</NlmUniqueID><ISSNLinking>0301-4851</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>6GC8A4PAYH</RegistryNumber><NameOfSubstance UI="C035276">eicosapentaenoic acid ethyl ester</NameOfSubstance></Chemical><Chemical><RegistryNumber>AAN7QOV9EA</RegistryNumber><NameOfSubstance UI="D015118">Eicosapentaenoic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.19.-</RegistryNumber><NameOfSubstance UI="D044943">Fatty Acid Desaturases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.3.-</RegistryNumber><NameOfSubstance UI="C115532">sphingolipid desaturase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D010367" MajorTopicYN="N">Arachis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002849" MajorTopicYN="N">Chromatography, Gas</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015118" MajorTopicYN="N">Eicosapentaenoic Acid</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044943" MajorTopicYN="N">Fatty Acid Desaturases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015202" MajorTopicYN="N">Protein Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Eicosapentaenoic acid</Keyword><Keyword MajorTopicYN="N">Peanut</Keyword><Keyword MajorTopicYN="N">Transgenic plants</Keyword><Keyword MajorTopicYN="N">Δ8-pathway</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>07</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>12</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30511300</ArticleId><ArticleId IdType="doi">10.1007/s11033-018-4476-1</ArticleId><ArticleId IdType="pii">10.1007/s11033-018-4476-1</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Science. 2000 Jan 14;287(5451):303-5</Citation><ArticleIdList><ArticleId IdType="pubmed">10634784</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2000 Jul 19;1486(2-3):219-31</Citation><ArticleIdList><ArticleId IdType="pubmed">10903473</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2000 Oct 16;159(1):7-19</Citation><ArticleIdList><ArticleId IdType="pubmed">11011088</ArticleId></ArticleIdList></Reference><Reference><Citation>Prog Lipid Res. 2001 Jan-Mar;40(1-2):1-94</Citation><ArticleIdList><ArticleId IdType="pubmed">11137568</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2001 Apr;160(5):889-898</Citation><ArticleIdList><ArticleId IdType="pubmed">11297785</ArticleId></ArticleIdList></Reference><Reference><Citation>J Lipid Res. 2001 Aug;42(8):1257-65</Citation><ArticleIdList><ArticleId IdType="pubmed">11483627</ArticleId></ArticleIdList></Reference><Reference><Citation>Prostaglandins Leukot Essent Fatty Acids. 2003 Feb;68(2):97-106</Citation><ArticleIdList><ArticleId IdType="pubmed">12538073</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Feb 8;361(9356):477-85</Citation><ArticleIdList><ArticleId IdType="pubmed">12583947</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnology. 1992;24:384-6</Citation><ArticleIdList><ArticleId IdType="pubmed">1422046</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2004 Jun;22(6):739-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15146198</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2004 Oct;16(10):2734-48</Citation><ArticleIdList><ArticleId IdType="pubmed">15377762</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2005 Aug;23(8):1013-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15951804</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2007 Dec;26(12):2071-82</Citation><ArticleIdList><ArticleId IdType="pubmed">17653723</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2008 Jun;27(6):1017-25</Citation><ArticleIdList><ArticleId IdType="pubmed">18320194</ArticleId></ArticleIdList></Reference><Reference><Citation>N Biotechnol. 2009 Oct 1;26(1-2):11-6</Citation><ArticleIdList><ArticleId IdType="pubmed">19501678</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2010 Apr;19(2):221-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19582587</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2009 Sep;7(7):602-10</Citation><ArticleIdList><ArticleId IdType="pubmed">19702754</ArticleId></ArticleIdList></Reference><Reference><Citation>Metab Eng. 2010 May;12(3):233-40</Citation><ArticleIdList><ArticleId IdType="pubmed">20004733</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Biotechnol. 2011 Apr;22(2):252-9</Citation><ArticleIdList><ArticleId IdType="pubmed">21144729</ArticleId></ArticleIdList></Reference><Reference><Citation>Endocr Metab Immune Disord Drug Targets. 2011 Sep 1;11(3):232-46</Citation><ArticleIdList><ArticleId IdType="pubmed">21831036</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Microbiol. 2011 Oct;63(4):387-91</Citation><ArticleIdList><ArticleId IdType="pubmed">21833666</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2013 Feb;11(2):157-68</Citation><ArticleIdList><ArticleId IdType="pubmed">23066823</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(11):e49165</Citation><ArticleIdList><ArticleId IdType="pubmed">23145108</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2013 Sep;97(17):7689-97</Citation><ArticleIdList><ArticleId IdType="pubmed">23229570</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2013 Nov;83(4-5):391-404</Citation><ArticleIdList><ArticleId IdType="pubmed">23839253</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2014 Jan;77(2):198-208</Citation><ArticleIdList><ArticleId IdType="pubmed">24308505</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2014 Jan 21;9(1):e85061</Citation><ArticleIdList><ArticleId IdType="pubmed">24465476</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2015 Dec;13(9):1264-75</Citation><ArticleIdList><ArticleId IdType="pubmed">25640865</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2016 Jul 19;11(7):e0158103</Citation><ArticleIdList><ArticleId IdType="pubmed">27433934</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2017 Jul 5;10(7):918-929</Citation><ArticleIdList><ArticleId IdType="pubmed">28666688</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2018 Jun;16(6):1107-1109</Citation><ArticleIdList><ArticleId IdType="pubmed">29337409</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 1997 Oct;15(10):401-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9351284</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Chenchen" sort="Wang, Chenchen" uniqKey="Wang C" first="Chenchen" last="Wang">Chenchen Wang</name></noRegion><name sortKey="Li, Xinzheng" sort="Li, Xinzheng" uniqKey="Li X" first="Xinzheng" last="Li">Xinzheng Li</name><name sortKey="Li, Xinzheng" sort="Li, Xinzheng" uniqKey="Li X" first="Xinzheng" last="Li">Xinzheng Li</name><name sortKey="Liu, Fengzhen" sort="Liu, Fengzhen" uniqKey="Liu F" first="Fengzhen" last="Liu">Fengzhen Liu</name><name sortKey="Liu, Fengzhen" sort="Liu, Fengzhen" uniqKey="Liu F" first="Fengzhen" last="Liu">Fengzhen Liu</name><name sortKey="Qing, Xiaohe" sort="Qing, Xiaohe" uniqKey="Qing X" first="Xiaohe" last="Qing">Xiaohe Qing</name><name sortKey="Qing, Xiaohe" sort="Qing, Xiaohe" uniqKey="Qing X" first="Xiaohe" last="Qing">Xiaohe Qing</name><name sortKey="Sun, Quanxi" sort="Sun, Quanxi" uniqKey="Sun Q" first="Quanxi" last="Sun">Quanxi Sun</name><name sortKey="Wang, Chenchen" sort="Wang, Chenchen" uniqKey="Wang C" first="Chenchen" last="Wang">Chenchen Wang</name><name sortKey="Yu, Mingli" sort="Yu, Mingli" uniqKey="Yu M" first="Mingli" last="Yu">Mingli Yu</name><name sortKey="Yu, Mingli" sort="Yu, Mingli" uniqKey="Yu M" first="Mingli" last="Yu">Mingli Yu</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Qi, Baoxiu" sort="Qi, Baoxiu" uniqKey="Qi B" first="Baoxiu" last="Qi">Baoxiu 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